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Image Search Results
Journal: Frontiers in Bioengineering and Biotechnology
Article Title: A novel method for the establishment of autologous skin cell suspensions: characterisation of cellular sub-populations, epidermal stem cell content and wound response-enhancing biological properties
doi: 10.3389/fbioe.2024.1386896
Figure Lengend Snippet: Proteomic analysis of the ACS secretome. Semiquantitative analysis using proteome arrays and quantitative detection by ELISA assays of soluble mediators secreted by ACS-derived cell populations cultured in serum-free medium at the indicated time points. Supernatants from cultures established from ACS were collected and analysed as detailed in the Methods. (A) Relative intensity values of densitometric analysis of ACS-secreted soluble factors using an angiogenesis array on days 1, 3 and 6 after isolation. (B) Relative intensity values of similar analysis using a cytokine proteome array on the same time-points post-isolation. (C) Detection of Interleukin-1 alpha, HMGB1 and Hsp90α in the ACS secretome by analyte-specific ELISA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, non-significance.
Article Snippet: Detection of analytes released in vitro from collected conditioned medium (at the indicated time points following culture initiation) from ACS-derived cultures was performed using an
Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Cell Culture, Isolation
Journal: Journal of Cerebral Blood Flow & Metabolism
Article Title: Non-classical monocytes promote neurovascular repair in cerebral small vessel disease associated with microinfarctions via CX3CR1
doi: 10.1177/0271678X231183742
Figure Lengend Snippet: Non-classical monocytes promote vascular function in cSVD. (a) Representative LSCI showing the temporal changes in the CBF of WT C and MDP mice up to 7 days post-cSVD. Analysis of CBF (b) at baseline (c) 24 h, (d) 3 days and (e) 7 days post-cSVD, shown as ipsilateral/contralateral (I/C) ratio. (f) Representative images of the Proteome Profiler mouse angiogenesis array membranes profiling expression of proteins implicated in vascular remodeling in the serum of CX3CR1 GFP/+ C and MDP mice 3 days post-cSVD. Analysis of the expression (optical density) of (g) CD105, (h) NOV/CCN3, (i) PDGF-AA and (j) VEGF, (k) serpin E1, (l) serpin F1, (m) thrombospondin 2 and (n) TIMP-4 in the serum of CX3CR1 GFP/+ C and MDP mice. Data are boxplot with min/max (n = 4–5 animals/group; n = 4 dots/experimental condition). I/C ratio = 1 indicates similar pattern in ipsilateral and contralateral hemispheres. *P < 0.05/**P < 0.01/***P < 0.001/ **** P < 0.0001 compared to WT C mice or CX3CR1 GFP/+ C mice (unpaired two-tailed t -test). Statistical summary is provided in (Supplementary Material 2). D, days.
Article Snippet: Blood was collected and allowed to clot in Eppendorf tubes for 2 h at RT and centrifuged at 2000×g for 20 min. Supernatant was collected and processed in a membrane-based
Techniques: Expressing, Two Tailed Test